Poster 124: Elevated Lipid Metabolites in Stored Clinical OCA Media are Associated with Chondrocyte Death

Objectives: A major limitation of osteochondral allografts (OCA) is the deterioration cartilage health associated with cell death during prolonged storage. It well established that chondrocyte viability in OCA a critical factor for repair and clinical outcomes, but little known about mechanisms contribute to Elevated bioactive lipid metabolites free fatty acids (FFAs) have been tissue damage various pathologies through lipotoxicity. However, levels effect FFA accumulation media storage are unknown. The purpose this study was determine if accumulate loss We hypothesized FFAs will over time adversely impact Methods: To elucidate potential decline storage, 21 fresh human hemi-condyle allograft tissues provided by US bank were analyzed after 7, 28, 68 days cold Targeted metabolomics analysis measured FFAs, primary (lipid hydroperoxide, ROOH) secondary (malondialdehyde, MDA) oxidation products. Chondrocyte using standard LIVE/DEAD assay confocal microscopy. Results: All acid significantly increased (Figure 1; p<0.05). ROOH higher at Day 28 No difference hydroperoxide products observed. declined both en face vertical cross-section 2) inversely correlated 3; Conclusions: elevated certain can alter function cause lipotoxicity other mechanisms. This work first identify product from OCA. concentrations (>100µM) induce directly viability. These findings provide important targets understanding why declines better optimize formulations improve graft health.